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Important: Normalase is not compatible with PCR - free workflows because it requires PCR amplification.
xGen™ DNA Library Prep
xGen™ cfDNA & FFPE DNA Library Prep
xGen™ ssDNA & Low-input DNA Library Prep
Key Features
(i.e., sample type, sample quality, applications)
(i.e., sample type, sample quality, applications)
General purpose DNA library prep kit
High conversion library prep kit for challenging samples (e.g. FFPE, cfDNA)
Library prep kit for single stranded DNA or ssDNA+dsDNA mixtures
Workflow Types
PCR-free and PCR-amplified
PCR-amplified
PCR-amplified
Fragmentation
Options for Mechanical and Enzymatic fragmentation
Mechanical
Mechanical
Input (ng)
Mechanical:
1 ng - 1 μg
Enzymatic:
100 pg - 1 μg
1 ng - 1 μg
Enzymatic:
100 pg - 1 μg
1 ng - 250 ng
10 pg - 250 ng
UMI-Based Error Correction Options
Single Read Family with UDI-UMI adapters
Single Read Family & Combined Read Family (Adapters with in-built UMIs included with kit)
None
xGen™ RNA Library Prep
xGen™ Broad-range RNA Library Prep
Key features
Fast workflow
Broad input range
Input
5 ng - 100 ng (mRNA, rRNA-depleted RNA, total RNA)
100 ng - 1 μg (total RNA into an upstream module)
100 ng - 1 μg (total RNA into an upstream module)
100 pg - 100 ng (mRNA, rRNA-depleted RNA, total RNA)
10 ng - 1 μg (total RNA into an upstream module)
10 ng - 1 μg (total RNA into an upstream module)
Sample Type
ribosomal RNA-depleted RNA
poly(A)-enriched mRNA
total RNA
formalin-fixed, paraffin-embedded (FFPE) RNA
poly(A)-enriched mRNA
total RNA
formalin-fixed, paraffin-embedded (FFPE) RNA
ribosomal RNA-depleted RNA
poly(A)-enriched mRNA
total RNA
formalin-fixed, paraffin-embedded (FFPE) RNA
RNA with low DV200 scores
poly(A)-enriched mRNA
total RNA
formalin-fixed, paraffin-embedded (FFPE) RNA
RNA with low DV200 scores
Time Required
~3.5 hours
~4.5 hours
Index Attachment
Fragmentation Method
Do you currently have issues with over-fragmenting of input DNA?
Select index(es) of interest
Select index(es) of interest
If you would like alternative full-length adapter designs, please visit the
Custom Adapter Configurator Tool
For more information, please contact Scientific Applications Support using the “Get Help” button below
For information on Exome sequencing, visit the
xGen™ Exome Hyb Panel v2
page.
Select all other xGen™ preconfigured hyb panel(s) for your application
Select all xGen™ preconfigured hyb panel(s) for your application
For information on these stocked panels, visit the
xGen™ Predesigned Hyb Panels
page. For designing custom hyb panels, visit the
xGen™ Custom Hyb Panels
page.
Summary
- Indexes:
- Indexes:
- Enzymatic Fragmentation:
- Unique Molecular Identifier:
- Deceleration Module:
- Index by Ligation:
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