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Next generation sequencing (NGS)

Next generation sequencing (NGS) technologies are an integral part of genetic research and discovery. The ability to generate large amounts of sequence data in a relatively short amount of time is helping to enable a wide range of genetic analysis applications and accelerate advances in research, clinical, and applied markets.

Critical to the success of obtaining high quality results in NGS experiments is the availability of high-quality tools to effectively prepare fragment libraries representing the original nucleic acid samples. From platform adapters to our portfolio of xGen™ products for targeted sequencing, IDT’s mission is to deliver innovative, high performing NGS products to enable you to increase your discovery power.

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Extraction

  • Beckman Coulter Life Sciences Genomics Reagents
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Sequencing

  • Illumina
  • Pacific Biosciences
  • Oxford Nanopore Technologies

NGS STARTS HERE

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Library preparation

In addition to targeted sequencing, IDT has library prep kits and NGS adapters available to support the workflows for high throughput sequencing. IDT library prep kits produce quality libraries from a range of sample input amounts. Unique molecular identifier (UMI) sequences can be incorporated during ligation enable a variety of deduplication and error correction strategies. Our range of adapter offerings contain a variety of indexing options including unique dual indexes (UDIs), which mitigate sample misassignment due to index hopping. These workflow components are compatible with our targeted sequencing offerings or can fit into workflows for other next generation sequencing applications.

Uses for library preparation include:

  • Whole genome sequencing (WGS)
  • PCR-free sequencing
  • Detection of germline, inherited single nucleotide polymorphisms (SNPs) and insertion/deletions (indels)
  • Low-frequency somatic variant detection of SNPs and indels
  • RNA-seq
  • Metagenomic sequencing
  • Methyl-Seq
 
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Quality
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Scalability
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Flexibility

Hybridization capture

Hybridization capture is a targeted next generation sequencing method that uses long, biotinylated oligonucleotide baits (probes) to hybridize to the regions of interest. It is particularly helpful when genotyping, detecting rare variants, and exome sequencing.  xGen hybridization capture panels consist of 5′ biotin–modified oligonucleotide probes that are individually synthesized. Individual probes are made in large lots and then aliquoted to maintain reproducibility. This rigorous manufacturing process gives xGen hybridization capture panels a unique advantage over array-derived pools, in which missing or truncated probes cannot be identified before sequencing. Using IDT proprietary synthesis methods, even probes with high GC and AT content are appropriately represented. In addition to providing stocked hyb capture panels, we can design custom panels for a given experiment. xGen Blocking Oligos and the xGen Hybridization and Wash Kit round out the workflow. We work with you to tailor products to your needs. 

Uses for hybridization capture include:

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Speed
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Cost
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Quality

Amplicon sequencing

Our proprietary rhAmp™ PCR technology drives the rhAmpSeq™ amplicon sequencing system. Using RNA-base–containing blocked primers (rhAmp primers), this technology harnesses the intrinsic properties of the RNase H2 enzyme to recognize and cleave DNA:RNA duplexes. rhAmp PCR is thus a powerful tool for increasing amplification specificity and minimizing the biggest limitation of PCR multiplexing—primer dimers.

The rhAmpSeq system combines the performance advantages of rhAmp PCR with a fast, easy-to-use workflow that requires only 2 PCR amplification steps to generate amplicon libraries for Illumina sequencing platforms. Coupled with our best-in-class DNA and RNA manufacturing, the rhAmpSeq system offers high-quality, targeted, sequencing results quickly and cost-effectively. Use the rhAmpSeq Sample ID Panel for easy, reliable human sample identification or design your own with the rhAmpSeq Design Tool

Uses for amplicon sequencing include:

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Ease-of-use
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Speed
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On-target

Adapters for next generation sequencing platforms »

Comprehensive offering of custom and catalog adapters for any NGS platform from the leader in nucleic acid manufacturing. Choose from a variety of barcoding and error correcting strategies, including designs with unique dual index (UDI) barcodes and unique molecular indentifiers (UMIs).

NGS library preparation kits »

From the Lotus™ DNA Library Prep Kit’s flexible workflow to the xGen Prism DNA Library Prep’s superior performance with difficult samples, we have the right kit for your application.

xGen products for target capture »

A suite of high performing enrichment probes, blockers, and workflow reagents for targeted sequencing. The xGen product portfolio enables unparalleled target coverage and uniformity, even across difficult genomic regions containing high GC content.

rhAmpSeq products for amplicon sequencing »

The rhAmpSeq system enables highly accurate amplicon sequencing on Illumina® NGS platforms. Whether you are investigating thousands of targets or a few, the fast and easy rhAmpSeq workflow generates NGS-ready amplicon libraries for deep, targeted resequencing.