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SameDay DNA Oligos

Custom DNA oligos-manufactured and shipped the same day you order

SameDay Oligos are standard, desalted DNA oligos, delivered in tubes using our fastest shipping method. Place your order before the specified deadline to receive your oligos the following business day.

Ordering

  • Receive your custom oligos the next business day after placing your order
  • Tested by ESI-MS prior to shipment*-expedited without compromising quality
  • No minimum or maximum order requirements
  • Choose formulation for shipment-dry or hydrated in IDTE, pH 8

* With the exception of mixed base oligos, which will result in multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

SameDay Oligos

Shipped dry or resuspended to 100 µM in IDTE buffer, pH 8.0. Delivered in tubes. To meet the ordering deadline, orders for SameDay Oligos must be placed online.

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Product Details

SameDay Oligos are standard desalted DNA oligos, delivered in tubes using our fastest shipping service available. If you place your order before the day's deadline (see below), you will receive your oligos on the following business day.

Every DNA Oligo you receive will be deprotected and desalted to remove small molecule impurities. In addition, your oligos will be quality controlled via proprietary ESI-mass spectrometry methods* and quantitated twice by UV spectrophotometry for precise yield measurements.

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

SameDay DNA Oligos must be ordered online and are no longer available by email request.

Specifications

  • 8 nmol minimum guarantee (sufficient for >250 PCR reactions)
  • 15−45 DNA bases; no modifications or modified bases
  • Each oligo quality controlled by ESI mass spectrometry*
  • Available in tubes only, dry or hydrated in IDTE buffer, pH 8.0
  • Deprotected and desalted
  • Additional shipping and handling fee for expedited delivery

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

Order deadlines

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United States

Order online by 3:00 pm ET

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Europe

Order online by 1:00 pm CET

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Singapore

Order online by 10:00 am SGT

Product Data

IDT's long-standing reputation as a leader and pioneer in custom oligo manufacturing is largely due to our vertically integrated proprietary synthesis platforms. Nearly every component of our manufacturing process is designed and developed in-house, including specialized synthesizers that can accommodate the most demanding oligo requests, and high-throughput automation systems that help ensure your oligos reach you when you need them. We do not rely on third-party manufacturers for any of the machines or chemical reagents used for synthesis, allowing us to monitor and refine instruments and reagents with ease. Our ability to control these variables means we can consistently produce quality oligos.

An important measure of quality for any oligo manufacturer is coupling efficiency, because this directly affects the amount of full-length product you receive. Our manufacturing processes result in high coupling efficiencies (Figure 1) and are standardized across our global manufacturing sites (Figure 2), meaning no matter where in the world you are conducting your research, an IDT oligo is an IDT oligo. Our commitment to quality and consistency results in a reliable product that helps drive your research forward.

Figure 1. IDT proprietary platforms have a better coupling efficiency than other suppliers, which provides more full-length oligonucleotides in your order. Small increases in coupling efficiency (≤1%) result in measurable increases in full-length product yield. The curves represent the theoretical yield for different lengths of oligos based on a coupling efficiency of 99.4% (IDT oligos, n = 126) and 99.1% (other supplier, n = 134 from three different suppliers) using the formula, percent full length product = (eff )(n-1)*100 where eff = coupling efficiency (for example, 99.4% = 0.994) and (n–1) is the number of coupling reactions needed to make an oligo of length n.

Figure 2. IDT tracks coupling efficiency at our manufacturing sites to ensure consistent quality. Many variables can lead to reduced oligo quality, including ambient temperature, humidity, and input reagents. Monitoring of coupling efficiency at IDT manufacturing sites ensures that we provide our customers with high quality products. A quarterly average is shown (n = 16 per manufacturing site).

Frequently asked questions

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.

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