Processing
CRISPR genome editing
Use of the CRISPR (clustered regularly interspaced short palindromic repeats) and associated Cas9 enzyme for genome editing has been a major technological breakthrough, making genome modification in cells or organisms faster, more efficient, and more robust than previous genome editing methods. The Alt-R CRISPR-Cas9 System is an optimized genome editing solution that outperforms other CRISPR approaches for producing on-target, double-stranded DNA breaks.
We have also developed an alternative Alt-R CRISPR-Cas12a (Cpf1) System to open up CRISPR editing to additional areas in genomes.
The Alt-R CRISPR Systems were developed through comprehensive research on each component of the CRISPR-driven, double-stranded break generation critical for gene disruption and DNA insertion by homologous recombination.
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ORDER NOWAlt-R CRISPR-Cas9 System »
Efficient CRISPR reagents based on the commonly used Streptococcus pyogenes Cas9 system for lipofection or electroporation experiments. Protospacer adjacent motif (PAM) = NGG.
Alt-R CRISPR-Cas12a (Cpf1) System »
For additional target sites or for targeting AT-rich regions, use the Acidaminococcus sp. BV3LC CRISPR-Cas12a system in electroporation experiments. Protospacer adjacent motif (PAM) = TTTV. The new Alt-R Cas12a (Cpf1) Ultra also can recognize many TTTT PAM sites in addition to TTTV motifs, increasing target range for genome editing studies.
CRISPR nucleases »
Recombinant, high-purity Cas9 and Cas12a (Cpf1) endonucleases for genome editing experiments.
CRISPR custom guide RNAs »
Custom guide RNAs ideal for prime editing (pegRNA) projects, CRISPR-Cas13 applications, and most alternative CRISPR-Cas systems.
Alt-R HDR Donor Oligos »
Single-stranded DNA oligos specifically built for your homology-directed repair (HDR) experiments. Designed and optimized through extensive wet-bench testing, they are ideal for introducing point mutations and short insertions.
Alt-R HDR Donor Blocks »
Double-stranded DNA fragments specifically built for your homology-directed repair (HDR) experiments. IDT dsDNA templates offer a cost-effective, high-fidelity option to reduce the amount of downstream screening via higher HDR rates and lower unintended blunt integrations. Alt-R HDR Donor Blocks are ideal for making large insertions or genomic changes.
rhAmpSeq CRISPR Analysis System »
An end-to-end solution to design, deploy, and analyze next generation sequencing data for on- and off-target interrogation after your CRISPR experiment.
Genome editing detection »
T7 endonuclease I (T7EI) mismatch cleavage assay for detection of on-target editing, known off-target events, and estimation of genome editing efficiency in cultured cells.
Quick comparison of CRISPR genome editing using Cas9 vs. Cas12a (Cpf1)
| Cas9 system | Cas12a system | |
|---|---|---|
| Applications | General genome editing |
|
| Ribonucleoprotein components |
|
|
| Variants |
|
|
| Cas9 crRNA:tracrRNA (option 1) | crRNA
tracrRNA
| — |
| Cas9 sgRNA (option 2) |
| — |
| Cas12a crRNA | — |
|
| CRISPR enzyme |
|
|
| DNA cleavage |
|
|
| PAM sequence† | NGG |
|
| Current recommendations for Alt-R RNP delivery |
|
|
* Molecular weight of Alt-R nuclease
† N = any base; V = A, C, or G
This comparison table is available for download (see page 2).
Optimizing donor templates for HDR
Download this expert guide to using Megamer ssDNA Fragments to enhance your HDR success
GET THE GUIDECRISPR validation with rhAmpSeq™ technology
Check your editing accuracy and efficiency
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IDT does not sell gene therapy kits and nothing sold by IDT should be construed as a gene therapy kit. Customers should not use any IDT products for self-administration.