Efficient CRISPR reagents based on the commonly used Streptococcus pyogenes Cas9 system for lipofection or electroporation experiments. Protospacer adjacent motif (PAM) = NGG.
For additional target sites or for targeting AT-rich regions, use the Acidaminococcus sp. BV3LC CRISPR-Cas12a system in electroporation experiments. Protospacer adjacent motif (PAM) = TTTV. The new Alt-R Cas12a (Cpf1) Ultra also can recognize many TTTT PAM sites in addition to TTTV motifs, increasing target range for genome editing studies.
Recombinant, high-purity Cas9 and Cas12a (Cpf1) endonucleases for genome editing experiments.
Custom guide RNAs ideal for prime editing (pegRNA) projects, CRISPR-Cas13 applications, and most alternative CRISPR-Cas systems.
Single-stranded DNA oligos specifically built for your homology-directed repair (HDR) experiments. Designed and optimized through extensive wet-bench testing, they are ideal for introducing point mutations and short insertions.
Double-stranded DNA fragments specifically built for your homology-directed repair (HDR) experiments. IDT dsDNA templates offer a cost-effective, high-fidelity option to reduce the amount of downstream screening via higher HDR rates and lower unintended blunt integrations. Alt-R HDR Donor Blocks are ideal for making large insertions or genomic changes.
An end-to-end solution to design, deploy, and analyze next generation sequencing data for on- and off-target interrogation after your CRISPR experiment.
T7 endonuclease I (T7EI) mismatch cleavage assay for detection of on-target editing, known off-target events, and estimation of genome editing efficiency in cultured cells.
|Cas9 system||Cas12a system|
|Applications||General genome editing|
|Cas9 crRNA:tracrRNA (option 1)|
|Cas9 sgRNA (option 2)||—|
|Current recommendations for Alt-R RNP delivery|
* Molecular weight of Alt-R nuclease
† N = any base; V = A, C, or G
This comparison table is available for download (see page 2).
IDT does not sell gene therapy kits and nothing sold by IDT should be construed as a gene therapy kit. Customers should not use any IDT products for self-administration.