Ultramer™ DNA Oligonucleotides

Ultramer DNA Oligos are long, single- and double-stranded synthetic DNA sequences. They are manufactured with an optimized synthesis platform that achieves consistent coupling efficiencies over 99.5%. With their increased length and purity, Ultramer DNA oligos have increased utility in a variety of molecular biology applications, including (but not limited to):

• Site-directed mutagenesis—used to efficiently and quickly generate large insertions, deletions or change stretches of sequence identity
• In vitro transcription—templates for the synthesis of RNA
• DNA-directed RNAi (ddRNAi)—hairpins are cloned into an expression vector, such as the GeneClip™ U1 Hairpin Cloning System (Promega)
• qPCR—as DNA standards
• CRISPR genome editing—as HDR templates

Every Ultramer DNA Oligo you receive will be deprotected and desalted to remove small molecule impurities. In addition, your oligos will be sequence-verified via proprietary ESI-mass spectrometry methods*, and quantitated twice by UV spectrophotometry for accurate yield measurements.

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

Available modifications for Ultramer oligos

IDT proprietary DNA synthesis equipment permits rapid, high quality synthesis of nucleic acids. This platform is adjusted to a lower throughput mode which uses an "extra rich" synthesis cycle for long oligos. Along with this refined synthesis cycle, Ultramer DNA Oligos use a unique solid support that is specifically optimized to synthesize low-yield, high-quality oligos up to 200 bases in length. Overall, these improvements to our already established manufacturing methods allow you to acquire longer, purer oligos for your research. Figure 1 illustrates how we attain the exceptional quality of Ultramer DNA Oligos.

Our proprietary ESI-mass spectrometry methods allow us to confidently provide quality control documentation for your oligos up to 200 bases in length*. These QC traces, such as the examples shown in Figure 2, are available online free of charge.

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

Application guides

Brochures and flyers

Frequently asked questions

OligoAnalyzer™ tool

Identify oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches

UNAFold tool

Perform secondary structure analysis

Dilution Calculator

Find the volume needed to dilute your oligo stock to a lower concentration

Resuspension Calculator

Find the volume needed to resuspend a dry oligo to a desired concentration

GMP and OEM

If you require oligos that are approved for use in molecular diagnostic applications, or if you are interested in our third-party manufacturing services, please click here.