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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What concentrations are the primers and probe in the PrimeTime® qPCR Assay?

All PrimeTime® qPCR Assay primers and probe are shipped dried down. The amount of primer and probe shipped depends on the assay scale ordered. The Mini scale is available only with probe containing 5' FAM™ dye, internal ZEN™ quencher, and 3' Iowa Black® FQ, and is sent at a fixed amount of 1 nmole primers (each) and 0.5 nmole probe. The Standard (500-20 µL reactions) and XL (2500-20 µL reactions) Assay scales have options for primer-to-probe ratio (ranging from 1:1 to 4:1), and a variety of dye/quencher combinations are available.