Find your correct clone with minimal screening
Using IDT gene fragments will reduce the time and expense of screening colonies compared to fragments from other suppliers (Table 2). Cloning efficiency is affected by many factors, including the cloning method used, the stability of the cell line and plasmid, vector preparation, and toxicity or stress from expression of coding sequences. The values in Table 2 represent typical screening numbers needed when using a seamless assembly method, such as Gibson Assembly® (Synthetic Genomics) or NEBuilder® HiFi assembly (New England BioLabs), and when issues mentioned above are not significant contributors to error or selection.
Table 2. Approximate number of colonies to screen for a 90% chance of getting a correct clone.