Processing
Double-stranded DNA (dsDNA) fragments
DNA fragments for synthetic biology and molecular biology applications. IDT offers a variety of reliable double-stranded solutions to meet your experimental needs.
Overview
IDT provides high-quality, high-fidelity, double-stranded gene fragments and genes, that are available for a variety of workflows and applications such as protein evolution, gene construction, and antibody engineering workflows, and more.
- Trusted for purity and quality
- Adaptable and flexible to fit your workflow and budget
- Reliable turnaround time
Design
Build
Test
Use Table 1 to help choose the best double-stranded DNA product for your experiment.
Table 1. Comparison of IDT Gene Fragments.
| eBlocks Gene Fragments | gBlocks Gene Fragments | gBlocks HiFi Gene Fragments | |
|---|---|---|---|
| Category | Linear dsDNA fragments | Linear dsDNA fragments | Linear dsDNA fragments |
| Available lengths (bp) | 300–1500 | 125–3000 | 1000–3000 |
| Median Error Rate | 1:5000 | 1:5000 | 1:12,000 |
| Estimated shipping time (business days) | 1–3 | 2–8* | 6–10 |
| Yield | 200 ng | 250–1000 ng | 1000 ng |
| Format | Plate | Tube/Plate | Tube |
| Application | High-throughput screening and antibody discovery | Gene construction and controls | Pathway design and large constructs |
*This estimated shipping time is for tubes only. Plates estimated ship date is 10-15 business days.
Find your correct clone with minimal screening
Using IDT Gene Fragments may reduce the time and expense of screening colonies compared to fragments from other suppliers. Cloning efficiency is affected by many factors, including the cloning method used, the stability of the cell line and plasmid, vector preparation, and toxicity or stress from expression of coding sequences. The values in Table 2 represent typical screening numbers needed when using a seamless assembly method, such as Gibson Assembly® (Codex DNA) or NEBuilder® HiFi assembly (New England BioLabs), and when issues mentioned above are not significant contributors to error or selection.
Table 2. Approximate number of colonies to screen for a 90% chance of getting a correct clone.
| Sequence length (bp) | eBlocks Gene Fragments | gBlocks Gene Fragments | gBlocks HiFi Gene Fragments |
|---|---|---|---|
| 500–900 | 2 | 2 | N/A* |
| 901–999 | 3 | 3 | |
| 1000–1500 | 3 | 3 | 2 |
| 1501–3000 | N/A* | 4 | 2 |
* N/A = not applicable (IDT Gene Fragments are not available at the lengths indicated)
Resources
Are you looking to optimize your sequences? The Codon Optimization Tool provides the best sequence option by screening and filtering sequences to lower complexity and minimize secondary structures.
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