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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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How do I dilute and store PrimeTime™ qPCR Probes?
We recommend resuspending PrimeTime qPCR Probes in TE (10 mM Tris pH 8.0, 0.1 mM EDTA). Alternatively, molecular grade water can be used. IDT recommends storing in a -20ºC freezer.
We find it convenient to initially make a 100 µM stock solution (which should be thawed relatively infrequently). Adding a volume of TE (µL) equal to 10 x # nmol DNA present in the tube (as noted on the spec sheet provided with the oligo) will produce a stock solution at this concentration.