The kit does not include primers or PCR reagents for quantification of gene silencing. Separately, we offer primer sets for targeting human or mouse HPRT for use in SYBR® Green–based qPCR assays to monitor RNAi function following transfection with the HPRT-S1 Positive Control DsiRNA.
For other human, mouse, or rat targets, consider using a Predesigned PrimeTime® qPCR Assay. For additional species, you can easily design a custom PrimeTime qPCR Assay using our free, online PrimerQuest® Tool.
The TriFECTa Kit includes: