Your product is now available from Integrated DNA Technologies.
Many of the Swift products you have grown to love are now part of our new complete portfolio, xGen™ NGS. Through this new partnership we are pleased to offer you comprehensive next generation sequencing solutions.
xGen NGS—made for you.
Unsure of what products are available? Or, perhaps you’d like guidance on which products are compatible? If so, try our xGen NGS Solutions Builder Tool today.
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Do annealed double-stranded oligos need to be modified prior to ligation into the appropriate restriction site of my cloning vector?
Our chemical synthesis results in a hydroxyl group on both the 3' and 5' terminus of the oligo. DNA ligase requires a 5' phosphate and a 3' hydroxyl group for activity, so a 5' phosphate will need to be added if the intent is to ligate the oligo. This can be requested upon order using the modification code /5Phos/ in the sequence of your oligo. Alternatively, this modification can be added enzymatically through a Polynulcleotide Kinase (PNK) reaction.