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Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Are there any special requirements for the handling and storage of my labeled probes?
Labeled probes should, for the most part, be handled as other custom oligonucleotide sequences. We recommend resuspending probes in TE buffer (10 mM Tris pH 8.0, 0.1 mM EDTA), such as or IDTE. Non-DEPC treated nuclease‑free water can be used, although it can limit shelf life. Ideally, for each resuspended probe, create a stock and several aliquots and store them all at –20°C. For long term oligo storage, temperature is the most important factor to consider.
For long term storage, whether oligos are dried down, or resuspended in water (non-DEPC treated) or TE buffer, it is optimal to store them frozen.
For room temperature or 4°C storage, oligos that are resuspended in TE buffer are more stable than dry oligos. Oligos that are stored in water are the least stable. At 4°C oligos stored under all of these conditions are stable for at least 60 weeks.