Combine NGS power with PCR simplicity for your next amplicon sequencing project
IDT’s rhAmpSeq™ technology is a PCR-based enrichment method for use on Illumina’s next generation sequencing platforms. The foundation of this product is IDT’s RNase H2 amplification technology. Upon hybridization of the primers to their specific target site, the RNase H2 enzyme cleaves the primer upstream of the RNA base. The cleavable, blocked primers are ideal for applications requiring large, multiplexed, PCR primer panels to help reduce the formation of primer dimers. Applications for this technology include detection of genetic markers in agricultural samples, detection of mutational hot spots relevant to oncology, and evaluation of on- and off-target genomic editing by CRISPR-Cas9. Custom rhAmpSeq Panels can be designed for any organism with a known genome. This webinar discusses the theory behind this technology and highlights the use of the rhAmpSeq system for identifying on- and off-target gene editing via CRISPR-Cas9.