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Technical reports

Comprehensive discussions of nucleic acids and their properties, calculations, strategies, techniques, and applications.

For overviews of common biotechnology concepts and methods, see our biotech basics.

gBlocks gene fragments as qPCR standards (361 KB)

gBlocks gene fragments as qPCR standards (361 KB)

Troubleshooting polyacrylamide gel electrophoresis (PAGE) (442 KB)

Troubleshooting polyacrylamide gel electrophoresis (PAGE) (442 KB)

In vitro nuclease detection (416 KB)

In vitro nuclease detection (416 KB)

Fluorescence and applications of fluorescent oligonucleotides (703 KB)

Fluorescence and applications of fluorescent oligonucleotides (703 KB)

Fluorescence quenching by proximal G bases (202 KB)

Fluorescence quenching by proximal G bases (202 KB)

Spectra of DNA-conjugated fluorescent dyes (228 KB)

Spectra of DNA-conjugated fluorescent dyes (228 KB)

Introducing antisense oligonucleotides into cells (420 KB)

Introducing antisense oligonucleotides into cells (420 KB)

RNA interference and DsiRNAs (249 KB)

RNA interference and DsiRNAs (249 KB)

Dicer design rules for RNAi (153 KB)

Dicer design rules for RNAi (153 KB)

Identification of antisense oligonucleotide motifs (240 KB)

Identification of antisense oligonucleotide motifs (240 KB)

Designing antisense oligonucleotides (386 KB)

Designing antisense oligonucleotides (386 KB)

Strategies for attaching oligonucleotides to solid supports (667 KB)

Strategies for attaching oligonucleotides to solid supports (667 KB)

Ultramer Oligonucleotides (519 KB)

Ultramer Oligonucleotides (519 KB)

Quantification of oligonucleotides with NanoDrop instruments (664 KB)

Quantification of oligonucleotides with NanoDrop instruments (664 KB)

Calculating Tm for oligonucleotide duplexes (215 KB)

Calculating Tm for oligonucleotide duplexes (215 KB)

Chemical synthesis of oligonucleotides (540 KB)

Chemical synthesis of oligonucleotides (540 KB)

Mass spectrometry analysis of oligonucleotides (383 KB)

Mass spectrometry analysis of oligonucleotides (383 KB)

Capillary electrophoresis of oligonucleotides (418 KB)

Capillary electrophoresis of oligonucleotides (418 KB)

Breaking PCR (337 KB)

Breaking PCR (337 KB)

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