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Target next generation sequencing with amplicon sequencing

rhAmpSeq™ Amplicon Sequencing System

Webinar summary: Dr Scott Rose, IDT Director of Enzyme Development, presents the rhAmpSeq Amplicon Sequencing System and its applications.

The rhAmpSeq system is a novel, innovative suite of reagents for targeted amplicon sequencing on Illumina next generation sequencing platforms. It can be multiplexed at high levels and has a simple workflow with 2 PCR steps. The rhAmp™ PCR chemistry greatly reduces primer dimers and other non-specific amplification, leading to better results with high on-target rates and excellent uniformity.

The rhAmpSeq system can be used for a variety of applications, from targeted genotyping-by-sequencing (GBS) in agricultural biotech to detection of disease-associated variants. The panel design is flexible, allowing you to add to select subsets from existing panels.

In the webinar below, rhAmpSeq targeted amplicon sequencing system: Combine NGS power with PCR simplicity for your next amplicon sequencing project, Dr Scott Rose presents details of the rhAmpSeq methodology and applications. One aspect of the methodology that supports maximum design success is the use of degenerate bases. Large numbers of single nucleotide polymorphisms (SNPs) near the target region can make primer design challenging. Incorporating degenerate bases into the primers prevents allele dropout. Dr Rose demonstrates how degenerate bases can benefit your experiment.

The webinar highlights one application of the rhAmpSeq system: identification of CRISPR-Cas9 gene edits. After CRISPR editing, on- and off-target editing events must be quantified. Dr Rose details how the rhAmpSeq system can be used to validate results from the HiFi Cas9 system and walks you through the off-target analysis. He explains how the rhAmpSeq system reveals improvements in homology-directed repair (HDR) rate when CRISPR is performed using Alt‑R™ modified, single-stranded oligodeoxynucleotides. Lastly, he reviews advantages of the rhAmpSeq system’s high-throughput protocol as well as other features and performance.

Watch the recorded webinar below to find out how the technology works and how it can improve your sequencing experiments.

Published Oct 22, 2019