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Precise genome editing with A.s. Cas12a (Cpf1) Ultra: A mutant protein with the reliability of S.p. Cas9
IDT staff scientist Dr Chris Vakulskas will talk about the development of a mutant protein, A.s. Cas12a (Cpf1) Ultra, which has dramatically improved cleavage to a level that rivals S.p. Cas9 without altering its PAM specificity.
The A.s. Cas12a (Cpf1) Ultra protein also demonstrates low-temperature cleavage activity superior to that of L.b. Cas12a, making it a universal Cas12a solution for genome editing in both animal and plant species.
Finally, IDT research scientist Bernice Thommandru will describe optimal HDR methods and reagents for maximizing HDR rates when using either A.s. Cas12a (Cpf1) Ultra or S.p. Cas9.